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NameMinsun Cha
Organization or InstitutionUniversity of Florida
TopicBiochemistry / Chem Bio.
Title

Investigation of charcoals, inorganic salts, and organic solvents for CoA dimer purification

Author(s)

Minsun Cha, Hyunjun Choe, and Jon D. Stewart

Author Institution(s)

University of Florida

Abstract

Coenzyme A (CoASH) plays an important role for many enzyme reactions acting as an acyl carrier and co-substrate. However, the high price of commercially available CoASH (2,600 USD/g with >85% purity) hampers the investigations of CoA-utilizing enzymes. CoASH could be produced through the disulfide reduction of CoA-S-S-CoA (CoA dimer), which is cost-effectively synthesized from pantethine by cascade enzyme reactions (pantetheine kinase, phosphopantetheine adenyltransferase, and dephospho-coenzyme A kinase). However, CoA dimer purification requiring an anion exchange column from all enzyme reaction components is still laborious. In an effort to remove the column purification step, various charcoals, inorganic salts, and organic solvents were investigated.